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                  <text>Colorado Parks and Wildlife (CPW) Planned Biological Monitoring and
Habitat Restoration Oversight in the Upper Colorado River
Background
The upper Colorado River is an iconic drainage in Colorado that has been severely impacted by
impoundments and reduced flows. Trans-basin and local water use divert approximately 67% of
the flow of the upper Colorado River and future projects will deplete flows further. There are
ongoing discussions on how to implement mitigation measures to reduce the impact of increased
trans-basin water diversions. One component is to reduce effects of the diversions by taking
Windy Gap Reservoir off channel and constructing a bypass around the reservoir. This would
reconnect the river and ameliorate various impacts of a large, on channel impoundment but would
not reduce the impacts of water withdrawals from the system. The planned bypass channel offers
a unique opportunity to evaluate the effects reconnecting the river through the reservoir as well as
investigate mitigation measures to offset the impacts of large water diversions on the ecology of
the river. The objectives of these projects are to evaluate the effectiveness of mitigation measures
in restoring and improving the ecological function of the upper Colorado River.
Invertebrate and Sculpin Evaluations
Current Project Description and Status
The current work on native sculpin and invertebrate species in the upper Colorado River is being
conducted by CPW aquatic research scientist Dan Kowalski. Previous work by CPW research
scientists identified ecological impacts of streamflow reductions and a main stem reservoir (Windy
Gap) on the invertebrates and fish of the river. Native mottled sculpin are currently rare or
extirpated immediately below the reservoir. The health of the invertebrate community declined
after the construction of Windy Gap Reservoir. There has been a 38% reduction in the diversity
of aquatic invertebrates from 1980 to 2011 and 19 species of mayflies, four species of stoneflies
and eight species of caddisflies had been extirpated from the sampling site below Windy Gap
(Erickson 1983, Nehring 2011). Previous work (Kowalski 2014) included mottled sculpin
sampling above and below the reservoir (as well as other impoundments of the upper Colorado
River) corroborated patterns of sculpin distributions and established that sculpin have been
functionally extirpated from the Colorado River below Windy Gap Reservoir. Once common in
this reach, sculpin are now absent for many miles downstream of the reservoir, but become
increasingly common downstream as depletions are offset by other reservoir releases to satisfy
downstream senior water rights.
Future Plans
A large amount of baseline data has already been collected previously under previous projects. To
evaluate the effects of the proposed bypass on invertebrates, sampling is planned to replicate the
historic sampling of invertebrates that occurred pre-impoundment and 25 years post impoundment
(Erickson 1983, Nehring 2011). A subset of sites from previous work will be selected to represent

�the most impacted areas below the reservoir as well as control sites. A before after control impact
study design will be employed for invertebrates using commonly accepted sampling methods.
Five replicate 0.086 m2 Hess samples will be taken from riffles at each study site.
Macroinvertebrate samples will be sorted and sub-sampled in the laboratory using a standard
USGS 300-count protocol, except that replicates will not be composited to allow for an estimate
of within site variation (Moulton et al. 2000). All organisms, except for chironomids and noninsects, will be identified to genus or species while chironomids will be identified to subfamily
and non-insects (e.g., oligochaetes, amphipods) identified to class. In addition to the analysis of
recent samples, data will be compared to previous collections six and 33 years previous.
To monitor any re-colonization of the river below Windy Gap Reservoir by the stonefly
Pteronarcys californica, methods developed by Nehring (2011) and Kowalski (2016) Heinold et
al. (in prep.) will be employed.
To evaluate the effects of the proposed bypass on native mottled sculpin, sampling is planned to
replicate the historic sampling that occurred pre- and post- impoundment (Erickson 1983,
Kowalski 2014, Nehring 2011). A before after control impact study design will be employed for
mottled sculpin using techniques developed on the Colorado River by previous work.
Colorado River Rainbow Trout Establishment
Current Project Description and Status
The current phase of the upper Colorado River rainbow trout reestablishment project, examining
fry stocking as a means to increase the adult rainbow trout population, began in 2013. Currently,
CPW aquatic research scientist Dr. Eric Fetherman is assigned to this project. Rainbow trout fry
have been stocked in to the upper Colorado River study section between Hitching Post Bridge and
the Sheriff Ranch every year since 2013. CPW aquatic biologist Jon Ewert, who is responsible for
fish population assessments in this area, simultaneously stocked the section from the Hot Sulphur
Springs office to Sunset Ranch with similar numbers of rainbow trout fry. To determine fry
survival rates, fry sites (four in the study section and three below Byers Canyon) have been
sampled once a month June through October. Fry infection rates were monitored by collecting
rainbow trout and brown trout fry samples for myxospore counts during fall sampling. Adult
population estimates have been conducted in the spring (late April-early May) to determine if fish
are recruiting to the adult population since 2013.
Future Plans
Fry stocking and evaluations will continue each year before and after the construction of the
bypass. Myxospore counts will be obtained from rainbow trout and brown trout before and after
construction of the bypass to determine if infection levels due to Myxobolus cerebralis, the parasite
that causes salmonid whirling disease, are changed due to reduction of available Tubifex tubifex
(the intermediate host of M. cerebralis) habitat.
Wild trout reproduction response from
construction of the bypass and related stream habitat work (connecting floodplain habitats and
reducing entrenchment) will be evaluated. Adult population estimates will be conducted to
determine if increased recruitment occurs after the construction of the bypass. During these

�estimates, genetic samples will be collected to determine the survival and recruitment of stocked
and naturally produced fry.
Stream Hydrology, Channel Function, and Habitat Evaluations
Current Project Description and Status
CPW hydrologist/ research scientist Eric Richer and CPW research scientist Matt Kondratieff are
involved in technical design assistance and review for the Windy Gap Bypass project and will
serve the same functions for the Upper Colorado River Habitat Project. This review and
oversight function for the entire segment of the upper Colorado River is important to integrate
the numerous aspects of stream habitat work in the drainage with Windy Gap bypass project.
Future Plans
The Windy Gap bypass project presents a unique opportunity to evaluate various aspects of
stable channel design and ecological restoration. The physical habitat characteristics for fish
species should be evaluated for post-project changes by monitoring embeddedness and substrate.
The project will create a new stream channel on a historic floodplain, and the new channel is
likely to experience some adjustments in morphology over time. Monitoring geomorphology
through repeated surveys for monumented cross-sections, the longitudinal profile of the new
bypass channel, and sediment composition will provide valuable information on channel stability
over time and inform maintenance needs. Ideally, bedload transport will be monitored to
determine if the bypass channel is achieving sediment continuity. As establishing riparian
vegetation will be critical for lateral channel stability, monitoring vegetation cover and
composition would help evaluate the need for additional vegetation work. If habitat structures are
used within the bypass channel, rapid assessment could be used to monitoring structural stability
and function which would inform maintenance needs.
Fish Movement and Habitat Use
If funding becomes available, additional work will be conducted in addition to the planned evaluation and
monitoring, which will more specifically evaluate use of the proposed Windy Gap bypass by fish and
invertebrates. RFID (PIT tagging) technology could be used to evaluate fish movement through the
bypass channel, adequacy of attraction flows from the bypass channel, and large-scale movement patterns
for various species in the Upper Colorado River watershed. Adult fish, including rainbow, brown trout,
and sculpins, will be marked upstream and downstream of Windy Gap Reservoir before construction of
the bypass. Portable PIT Tag arrays will be used to track movement of marked fish in the areas upstream
and downstream of the reservoir prior to and after construction of the bypass. Stationary PIT tag arrays
will be installed in the bypass to monitor movement through the newly constructed Windy Gap bypass
channel. Use of portable antennas will further allow the evaluation of fish use of habitat
treatments installed within the bypass. This work will be integrated with the ongoing monitoring of

invertebrates and fish to evaluate re-colonization in the bypass channel itself, as well as the
effects on species composition upstream and downstream of Windy Gap Reservoir.
References

�Erickson, R.C. 1983. Benthic field studies for the Windy Gap study reach, Colorado River,
Colorado, fall, 1980 to fall, 1981. Prepared for The Northern Colorado Water Conservancy District,
Municipal Sub-District.
Heinold, B.D., R.B. Nehring and D.A. Kowalski. In preparation. Estimating the density of benthic and
emerging Salmonflies (Pteronarcys californica) in Colorado rivers.
Kowalski, D.A. 2014. Colorado River aquatic resources investigations. Colorado Division of
Wildlife, Federal Aid in Sportfish Restoration, Project F-237-R21, Progress Report, Fort Collins.
Kowalski, D.A. 2016. Colorado River aquatic resources investigations. Colorado Division of
Wildlife, Federal Aid in Sportfish Restoration, Project F-237-R23, Progress Report, Fort Collins.
Moulton, S.R., II, Carter, J.L., Grotheer, S.A., Cuffney, T.F. &amp; Short, T.M. 2000. Methods of
analysis by the U. S. Geological Survey national water quality laboratory: processing, taxonomy,
and quality control of benthic macroinvertebrate samples. Open-File Report 00-212, U.S.
Geological Survey, Washington D.C.
Nehring, R.B. 2011. Colorado River aquatic resources investigations. Colorado Division of
Wildlife, Federal Aid in Sportfish Restoration, Project F-237R-18, Final Report, Fort Collins.

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              <text>The upper Colorado River is an iconic drainage in Colorado that has been severely impacted by impoundments and reduced flows. Trans-basin and local water use divert approximately 67% of the flow of the upper Colorado River and future projects will deplete flows further. There are ongoing discussions on how to implement mitigation measures to reduce the impact of increased trans-basin water diversions. One component is to reduce effects of the diversions by taking Windy Gap Reservoir off channel and constructing a bypass around the reservoir. This would reconnect the river and ameliorate various impacts of a large, on channel impoundment but would not reduce the impacts of water withdrawals from the system. The planned bypass channel offers a unique opportunity to evaluate the effects reconnecting the river through the reservoir as well as investigate mitigation measures to offset the impacts of large water diversions on the ecology of the river. The objectives of these projects are to evaluate the effectiveness of mitigation measures in restoring and improving the ecological function of the upper Colorado River.</text>
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